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. 2022 Nov 19;13(11):976. doi: 10.1038/s41419-022-05413-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Overexpression of Bnip3 does not affect reprogramming efficiency. Data shown are the mean ± SD, n = 3; NS no significant difference. B Bnip3 overexpression enhanced mitochondrial autophagy in reprogramming cells. GFP: LC3; RFP: Mitochondria. C Confocal fluorescence micrographs of reprogramming cells. SSEA-1-positive cells at reprogramming day 3 were sorted for staining with γH2AX and RAD51. Green, γH2AX; Red, RAD51; Blue, DAPI. D Statistical data for γH2AX foci in (C). Cells were randomly divided into 3 groups and the foci were counted. Data shown are the mean ± SD, n = 3; *, P < 0.05; Student’s t-test. E Statistical data for RAD51 and γH2AX colocalization foci/γH2AX foci in (C). F Confocal fluorescence micrographs of iPSCs. Green, γH2AX; Red, SOX2; Blue, DAPI. G Western blot detection of RAD51, γH2AX, p-AMPK, AMPK, and BNIP3 in wild-type or Bnip3-overexpressing iPSCs. Actin served as the loading control. H Quantifications of each protein expression in (G). Data are shown as mean ± SD, n = 3; ***, P < 0.001; NS no significant difference, Student’s t-test. I Acquired indels in indicated iPSC lines.