Table 2. Layout of the CCRD and the corresponding response values for the preparation of CFE-NLCs .
| No. | Independent variable | Dependent variable | ||||||
| X 1 (wt%) | X 2 (% total lipid content) | X 3 (wt%) | X 4 (wt%) | Y 1 (nm) | Y 2 | Y 3 (mV) | Y 4 (%) | |
| 1 | 6.75 | 30.00 | 6.50 | 1.38 | 59.25 ± 0.73a,h | 0.17 ± 0.02a | –11.93 ± 2.24a | 75.83 ± 5.54a,c,e,f |
| 2* | 6.75 | 30.00 | 3.50 | 1.38 | 99.98 ± 1.64b | 0.17 ± 0.01b,d | –10.48 ± 2.64a | 78.31 ± 1.17a,c,e,f |
| 3 | 6.75 | 30.00 | 3.50 | 0.13 | 99.29 ± 1.27b | 0.18 ± 0.03a | –10.05 ± 0.60a | 69.10 ± 4.13a,c,e,f |
| 4 | 3.50 | 15.00 | 2.00 | 0.75 | 111.13 ± 2.53b,c | 0.22 ± 0.02a | –11.16 ± 1.23a | 56.80 ± 7.85a,d,e |
| 5 | 6.75 | 30.00 | 3.50 | 2.63 | 104.00 ± 1.63b,c | 0.19 ± 0.02a | –12.70 ± 0.93a | 58.44 ± 1.78a,d,e |
| 6 | 0.25 | 30.00 | 3.50 | 1.38 | 76.48 ± 4.37a | 0.41 ± 0.27a | –14.89 ± 3.40a | 67.80 ± 0.14a,c,e,f |
| 7 | 6.75 | 30.00 | 0.50 | 1.38 | 489.33 ± 5.92d | 0.45 ± 0.21a,d | –14.79 ± 1.49a | 65.64 ± 3.94a,c,e |
| 8 | 10.00 | 15.00 | 5.00 | 2.00 | 151.43 ± 2.37e | 0.27 ± 0.01a | –6.68 ± 4.49b,c | 40.30 ± 1.88b,d,e |
| 9 | 13.25 | 30.00 | 3.50 | 1.38 | 240.00 ± 7.39f | 0.26 ± 0.06a | –9.85 ± 0.35a | 57.63 ± 0.41a,c,d,e |
| 10* | 6.75 | 30.00 | 3.50 | 1.38 | 111.20 ± 5.88b,c | 0.18 ± 0.03a | –10.89 ± 3.67a | 77.91 ± 4.18a,c,e,f |
| 11* | 6.75 | 30.00 | 3.50 | 1.38 | 106.11 ± 7.14b,c | 0.18 ± 0.02a | –10.72 ± 1.45a | 76.38 ± 1.79a,c,e,f |
| 12* | 6.75 | 30.00 | 3.50 | 1.38 | 95.76 ± 5.29b | 0.18 ± 0.02a | –10.07 ± 2.65a | 77.31 ± 1.87a,c,e,f |
| 13 | 10.00 | 45.00 | 5.00 | 0.75 | 120.57 ± 2.07c | 0.21 ± 0.02a | –6.16 ± 1.65b | 64.04 ± 2.21a,d,e |
| 14 | 3.50 | 15.00 | 2.00 | 2.00 | 120.50 ± 1.85c | 0.30 ± 0.06a | –12.95 ± 1.84a,c | 51.82 ± 3.99b,d |
| 15 | 10.00 | 45.00 | 2.00 | 2.00 | 243.00 ± 4.28f | 0.19 ± 0.05a | –12.73 ± 1.21a | 62.22 ± 1.65a,d,e |
| 16 | 10.00 | 45.00 | 5.00 | 2.00 | 117.00 ± 2.14c,g | 0.03 ± 0.00c | –10.70 ± 3.22a | 65.61 ± 5.39a,c,e |
| 17 | 10.00 | 15.00 | 5.00 | 0.75 | 121.39 ± 1.74c | 0.23 ± 0.01a | –5.76 ± 1.79b,c | 45.13 ± 3.01b,d |
| 18 | 3.50 | 45.00 | 5.00 | 0.75 | 53.25 ± 1.13h | 0.23 ± 0.03a | –8.58 ± 2.68a | 73.28 ± 1.97a,c,e,f |
| 19 | 10.00 | 15.00 | 2.00 | 0.75 | 459.25 ± 19.70i | 0.33 ± 0.13a | –12.71 ± 2.69a | 66.91 ± 7.10a,c,e |
| 20* | 6.75 | 30.00 | 3.50 | 1.38 | 99.76 ± 1.78b | 0.17 ± 0.03a,b | –10.69 ± 1.45a | 75.78 ± 5.18a,c,e,f |
| 21 | 10.00 | 15.00 | 2.00 | 2.00 | 456 ± 12.88i | 0.34 ± 0.09a | –12.01 ± 1.86a | 59.71 ± 7.72a,d,e |
| 22 | 3.50 | 15.00 | 5.00 | 2.00 | 76.48 ± 1.26a | 0.42 ± 0.02a | –7.99 ± 2.00a | 77.66 ± 1.20a,c,e,f |
| 23 | 6.75 | 0.00 | 3.50 | 1.38 | 147.00 ± 2.27e | 0.26 ± 0.01a | –10.85 ± 0.53a | 66.06 ± 4.79a,c,e |
| 24 | 3.50 | 45.00 | 2.00 | 2.00 | 82.21 ± 1.05a,b | 0.18 ± 0.02a | –15.06 ± 1.67a | 77.70 ± 4.50a,c,e,f |
| 25 | 10.00 | 45.00 | 2.00 | 0.75 | 235.29 ± 4.35f | 0.19 ± 0.04a | –8.02 ± 3.93a | 59.61 ± 1.38a,d,e |
| 26 | 3.50 | 15.00 | 5.00 | 0.75 | 88.85 ± 1.28a,b | 0.36 ± 0.03a | –7.71 ± 0.92a | 63.80 ± 5.41a,c,d,e |
| 27 | 3.50 | 45.00 | 2.00 | 0.75 | 90.94 ± 1.56a,b | 0.20 ± 0.02a | –12.94 ± 0.69a | 74.39 ± 5.91a,c,e,f |
| 28 | 3.50 | 45.00 | 5.00 | 2.00 | 57.68 ± 1.39a,h | 0.27 ± 0.04a | –10.62 ± 1.20a | 77.62 ± 5.40a,c,f |
| 29* | 6.75 | 30.00 | 3.50 | 1.38 | 96.78 ± 3.05b | 0.16 ± 0.09b,d | –10.87 ± 1.84a | 80.76 ± 3.12a,c,f |
| 30 | 6.75 | 60.00 | 3.50 | 1.38 | 124.13 ± 3.52c | 0.19 ± 0.02a | –13.58 ± 1.33a | 66.90 ± 1.60a,c,e |
Data are expressed as the mean ± SD (n = 3). Values sharing the same letter in each column do not exhibit statistically significant differences (P > 0.05).
* Center point.