Table 1.
The information of DuraClone IM T cell subsets Tube
| λ Excitation | 405 nm | 488 nm | 633 nm | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Product | PB | KrO | FITC | PE | ECD | PC5.5 | PC7 | APC | AF647 | AF700 | APC-AF700 | APC-AF750 |
| Phenotyping basic | – | CD45 | CD16 | CD56 | CD19 | – | CD14 | CD4 | – | CD8 | – | CD3 |
| B cell | IgM | CD45 | IgD | CD21 | CD19 | – | CD27 | CD24 | – | – | – | CD38 |
| T cell subsets | CD57 | CD45 | CD45RA | CCR7 | CD28 | PD1 | CD27 | CD4 | – | CD8 | – | CD3 |
| Dendritic cells | HLA–DR | CD45 | CD16 | * | – | CD1c | CD11c | Clec9A | – | – | CD123 | – |
| TCRs | TCRVδ2 | CD45 | TCRγδ | TCRαβ | HLA–DR | – | TCRVδ1 | CD4 | – | CD8 | – | CD3 |
| Treg | Helios | CD45 | CD45RA | CD25 | – | CD39 | CD4 | – | FoxP3 | – | – | CD3 |
| Granulocytes | CD15 | CD45 | CD294 | – | CD16 | CD33 | CD11b | PD–L1 | – | ** | CD62L | |
| Count | – | – | CD45 | Counting beads | 7–AAD | – | – | – | – | – | ||
PB Pacific Blue; KrO Krome Orange; FITC Fluorescein isothiocyanate; PE Phycoerythrin; ECD Phycoerythrin Texas Red-X; PC5.5 Phycoerythrin Cyanine 5.5; PC7 Phycoerythrin Cyanine 7; APC Allophycocyanin; AF647 Alexa Fluor 647; AF700 Alexa Fluor 700; APC-AF700 Allophycocyanin Alexa Fluor 700; APC-AF750 Allophycocyanin Alexa Fluor 750
*CD3/CD19/CD20/CD14/CD56; **, CD3/CD14/CD19/CD56