. Author manuscript; available in PMC: 2023 Jul 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2022 Apr 28;221:106121. doi: 10.1016/j.jsbmb.2022.106121

Table 2.

Variant-specific activity for the NAD⁺-dependent oxidation of S-tetralol and catalytic efficiencies of NAD(P)⁺-dependent reactions.

Variant	S-tetralol specific activity (μmol/min/ mg)	S-tetralol NAD⁺ k_cat/ K_m (min⁻¹mM⁻¹)	S-tetralol NADP⁺ k_cat/ K_m (min⁻¹mM⁻¹)
WT	3.8 ± 0.35	150 ± 11	270 ± 45
H5Q	3.7 ± 0.31	170 ± 13	320 ± 23
E77G	3.1 ± 0.24^**	180 ± 18	440 ± 40^**
K104D	2.4 ± 0.22^****	86 ± 8.1^***	320 ± 60
R258C	2.3 ± 0.14^****	120 ± 19^*	270 ± 26

For all analyses

p < 0.05

^**

p < 0.01

^***

p < 0.001

^****

p < 0.0001.