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. 2022 Oct 14;8(10):mgen000869. doi: 10.1099/mgen.0.000869

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© 2022 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 1. — The response to mechanical damage is affected in RNAi mutants. (a) Conidiation response to injury of the WT strain and RNAi mutants. Colonies of the indicated strains growing on PDA were damaged with a cookie mould and photographed 36 h later. The arrow indicates the areas of accumulation of conidia in response to injury (dark). (b) The graph shows the yield of conidia in response to injury of four biological replicates. (c) Hyphal regeneration 1 h after injury. Hyphae were stained with lactophenol cotton blue and examined by light microscopy. Arrows point to the new hyphae. Scale bar=10 µM. (d) Percentage of hyphae that regenerate after injury for the indicated strains. Three biological replicates were used, counting 50 hyphae per strain. In (b) and (d), a one-way ANOVA test, followed by Tukey honest significant differences was applied. Highlights in red indicate that there was a statistically significant difference when comparing the indicated strains with the WT (P<0.05).