Ptr isolate M4 ToxC1 locus and 2 kb flanking sequence region alignment to 12 other Ptr ToxC-producing isolates. The Biotrigo9-1 ToxC1 region has two large insertions within and downstream of ToxC1. Nucleotide sequence alignments (blue) between the ToxC1 region for Ptr isolates (top to bottom: M4, AR CrossB10, Biotrigo9-1, Pt-1C-BFP, V1, Ptr134, Alg215, CC142, EW306-2-1, EW7m1, SN0001A, SN0001C and SN0002B) (black lines). The M4 genes are shown as red arrows. The light blue alignment segments are regions of low identity among the isolates, while the crossed regions indicate a repeat region in each sequence.