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. 2022 Oct 10;8(10):mgen000872. doi: 10.1099/mgen.0.000872

Fig. 4.

Fig. 4.

The predicted protein sequence and structural alignments of SnTox3 and the isolate M4 protein KAF7577476. (a) Multiple protein sequence alignment of SnTox3, Ptt CAA9973983.1 (W1-1), Ptm CAA9957881.1 (SG1) and Ptr KAF7577476 (M4). The Kex2 motif conservation is shown boxed in red. Only four cysteine residues were conserved across the four species (black asterisks) and those not conserved (red asterisks) are shown below the alignment for P. nodorum and above for the Pyrenophora species. (b) The known 3D protein structure for SnTox3 (PDB 6WES). (c) The 3D structure for KAF7577476 as predicted by Phyre2. (d) Superimposed structural alignment (yellow) of SnTox3 and KAF7577476 with an RSDM of 1.14 Å.