SARS-CoV-2 infection induces AMPK phosphorylation.
(A) Immunoblots analyzing the phosphorylation of AMPK in SARS-CoV-2 infected Caco2 cells for early (1, 2, 6, and 12 h) and late (24, 48, 72, and 96 h) time points. Cells infected with 1 MOI of SARS-CoV-2 were harvested at various time intervals post-infection and analyzed by immunoblotting. (B) Quantitative representation of AMPK phosphorylation from three independent replicates. Densitometric values of phospho-AMPK bands were normalized against those of total-AMPK expression and GAPDH belonging to the corresponding samples and the values were plotted graphically.