TABLE 1.
Replication of L. pneumophila in lungs after intratracheal inoculation
| Mouse strain and experimental groupa | No. of bacteria in lungs (CFU/g)c after:
|
|||
|---|---|---|---|---|
| 2 h | 1 day | 2 days | 4 days | |
| A/J | ||||
| BCYE-grown bacteria | (2.0 ± 0.3) × 105 | (6.2 ± 0.3) × 106 | (9.4 ± 0.3) × 106 | (2.1 ± 0.4) × 107 |
| BCYE-grown bacteria + amoebal lysate | (9.6 ± 0.5) × 104 | (5.8 ± 0.4) × 106 | (8.4 ± 0.3) × 106 | (1.1 ± 0.2) × 107 |
| Amoebae infected with bacteria | (9.1 ± 0.4) × 104 | (6.9 ± 0.3) × 108b | (1.9 ± 0.3) × 108b | (4.3 ± 0.3) × 109b |
| Amoeba-grown bacteria | (1.6 ± 0.1) × 105 | (8.6 ± 0.4) × 108b | (2.7 ± 0.6) × 109b | (4.1 ± 0.5) × 109b |
| C57BL/6 Beige | ||||
| BCYE-grown bacteria | (3.6 ± 0.4) × 105 | (2.9 ± 0.3) × 106 | (6.1 ± 0.1) × 106 | (5.3 ± 0.4) × 106 |
| BCYE-grown bacteria + amoebal lysate | (1.4 ± 0.3) × 105 | (3.3 ± 0.4) × 106 | (7.4 ± 0.2) × 106 | (5.9 ± 0.2) × 106 |
| Amoebae infected with bacteria | (2.4 ± 0.3) × 105 | (5.8 ± 0.3) × 108b | (9.1 ± 0.3) × 108b | (9.9 ± 0.3) × 108b |
| Amoeba-grown bacteria | (1.8 ± 0.5) × 105 | (6.7 ± 0.4) × 108b | (9.7 ± 0.5) × 108b | (9.3 ± 0.4) × 108b |
| C57BL/6 | ||||
| BCYE-grown bacteria | (2.0 ± 0.3) × 105 | (2.1 ± 0.4) × 106 | (4.5 ± 0.4) × 106 | (4.0 ± 0.3) × 106 |
| BCYE-grown bacteria + amoebal lysate | (9.8 ± 0.2) × 104 | (3.0 ± 0.1) × 106 | (5.7 ± 0.3) × 106 | (6.3 ± 0.4) × 106 |
| Amoebae infected with bacteria | (2.1 ± 0.2) × 105 | (5.0 ± 0.3) × 108b | (8.3 ± 0.4) × 108b | (5.1 ± 0.2) × 108b |
| Amoeba-grown bacteria | (1.5 ± 0.5) × 105 | (6.1 ± 0.4) × 108b | (9.4 ± 0.5) × 108b | (6.5 ± 0.3) × 106 |
a
An inoculum of 106 bacteria was used for all experimental groups.
b
Significantly different (P < 0.05) from BCYE-grown L. pneumophila alone.
c
Data represent the means ± standard deviations of duplicate platings from seven mice.