Histological images in vivo. (A) H&E and Safranin O-fast green staining of NP in different groups. (Scale bar = 2 mm) (B) Tunel staining of NP in different groups (TUNEL positive: red). (Scale bar = 2 mm, 800 μm) (C) In vivo immunofluorescence images of NF-κB and IKB-α in the NP in different groups. (Scale bar = 2 mm) (D) Quantification of red areas from B. (E, F) Mean fluorescence intensity of NF-κB and IKB-α. The data were presented as mean ± SD. ns (no statistical significance), *p < 0.05, **p < 0.01 and ***p < 0.001 vs the puncture group; ns (no statistical significance), #p < 0.05, ##p < 0.01 and ###p < 0.001 vs the BMSCs + LM@Co-Q10 group. n = 3.