Figure 3.

Neutrophils from patients with MIS-C display a strong G-MDSC signature and are characterized by altered metabolism

(A) Volcano plot showing differentially expressed genes between MIS-C and pediatric healthy control samples. Color-coded points indicate genes that pass FDR correction with q < 0.05.

(B) Gene set enrichment analysis for the differentially expressed genes in (A). Bar lengths correspond to NES, with positive NES values indicating enrichment in MIS-C and negative values indicating enrichment in healthy controls. Asterisks denote significance as defined in the figure.

(C) GSEA enrichment plots for the NMF4 (Immature) and NMF5 (G-MDSC) signatures, the two NMF neutrophil signatures that passed FDR correction.

(D) Heatmap displaying scaled RNA-seq expression values for gene markers distinguishing pediatric acute COVID-19 and MIS-C. The color coding on the right of the heatmap indicates whether the gene was differentially expressed in MIS-C versus COVID-19 and healthy or COVID-19 versus MIS-C or healthy. Color-coded bars above indicate disease status, cardiac involvement for patients with MIS-C, course of treatment, and days since the administration of steroids.

See also Figures S2 and S3 and Tables S3, S4, and S5.