Reagent	Amount
10× FastAP Buffer	10 μL
SUPERase·In™ RNase Inhibitor	2 μL
TURBO™ DNase	1 μL
FastAP enzyme	8 μL
H2O	79 μL

Mix, add 100 μL/sample, incubate in Thermomixer at 1,200 rpm, 37°C, 15 min. Then, short spin and magnetic separation to remove Master Mix.