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| Reagent | Amount |
|---|---|
| 5× PNK pH 6.5 Buffer | 20 μL |
| 100 mM DTT | 1 μL |
| SUPERase⋅In™ RNase Inhibitor | 1 μL |
| TURBO™ DNase | 1 μL |
| T4 PNK enzyme | 7 μL |
| H2O | 45 μL |
