Lysis Buffer
Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 7.4 (1 M) | 50 mM | 5 mL |
NaCl (5 M) | 100 mM | 2 mL |
NP-40 (10% solution) | 1% | 10 mL |
SDS (10% solution) | 0.1% | 1 mL |
Sodium deoxycholate (10% solution) | 0.5% | 5 mL |
H2O | N/A | 77 mL |
Total | N/A | 100 mL |