Reagent	Amount
10× AffinityScript RT buffer	2 μL
100 mM DTT	2 μL
dNTPs (10 mM each)	2 μL
SUPERase⋅In™ RNase Inhibitor	0.3 μL
AffinityScript Multi-Temp RT	0.9 μL
H2O	2.8 μL

Mix, add 10 μL/sample, incubate in pre-heated PCR block at 55°C, 45 min.