| Reagent | Amount |
|---|---|
| iQ™ SYBR® Green Supermix | 5 μL |
| qPCR primer mix (10 μM each D502/D702) | 0.4 μL |
| H2O | 3.6 μL |
Mix, add 9 μL/sample in 96 well qPCR plate, add 1 μL diluted cDNA or controls, mix, seal.
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