| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 95°C | 3 min | 1 |
| Denaturation | 95°C | 15 s | 39 cycles |
| Annealing/Extension | 60°C | 30 s | |
| Melt/Dissociation | 55°C | 5 s | 1 |
| 95°C | 50 s |
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