Reagent | Final concentration | Amount |
---|---|---|
Tris, pH 7.5 (2 M) | 10 mM | 0.25 mL |
MgCl2 (1 M) | 5 mM | 0.25 mL |
KCl (3 M solution) | 100 mM | 1.67 mL |
Triton X-100 | 0.02% | 10 μL |
H2O | N/A | 47.82 mL |
Total | N/A | 50 mL |
Reagent | Final concentration | Amount |
---|---|---|
Tris, pH 7.5 (2 M) | 10 mM | 0.25 mL |
MgCl2 (1 M) | 5 mM | 0.25 mL |
KCl (3 M solution) | 100 mM | 1.67 mL |
Triton X-100 | 0.02% | 10 μL |
H2O | N/A | 47.82 mL |
Total | N/A | 50 mL |