Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 7.5 (1 M) | 500 mM | 25 mL |
MgCl2 (1 M) | 100 mM | 5 mL |
H2O | N/A | 20 mL |
Total | N/A | 50 mL |
Dilute 1:10 in H2O for 1× RNA Ligase Buffer.
Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 7.5 (1 M) | 500 mM | 25 mL |
MgCl2 (1 M) | 100 mM | 5 mL |
H2O | N/A | 20 mL |
Total | N/A | 50 mL |
Dilute 1:10 in H2O for 1× RNA Ligase Buffer.