Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 7.5 (1 M) | 100 mM | 10 mL |
NaCl (5 M) | 50 mM | 1 mL |
EDTA (0.5 M) | 10 mM | 2 mL |
Urea | 7 M | 42 g |
H2O | N/A | 87 mL |
Total | N/A | 100 mL |
Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 7.5 (1 M) | 100 mM | 10 mL |
NaCl (5 M) | 50 mM | 1 mL |
EDTA (0.5 M) | 10 mM | 2 mL |
Urea | 7 M | 42 g |
H2O | N/A | 87 mL |
Total | N/A | 100 mL |