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. 2022 Nov 13;17:100491. doi: 10.1016/j.mtbio.2022.100491

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Cell migration of hGFs on JEM membranes. (a and b) Representative images and quantitative analysis of hGFs on JEM(+), JEM(−), and control membranes at 24 h in the transwell assay. The scale bar in a is 200 μm, n = 6. (c) qRT-PCR analysis of migration-related gene expression (FAK, FN1, and COLI), n = 9. (d and e) Western blot analysis of migration-related proteins (FAK, FN1, and COL I) in hGFs on JEM(+), JEM(−), and control membranes at 24 h, n = 6. ∗p < 0.05, compared with Control; #p < 0.05, compared with JEM(+). Statistically significant differences (p < 0.05) were measured using a one-way analysis of variance (ANOVA) combined with the Student–Newman–Keuls (SNK) multiple comparison post hoc test.