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. 2022 Nov 7;13:1030920. doi: 10.3389/fphys.2022.1030920

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Copyright © 2022 Pioner, Santini, Palandri, Langione, Grandinetti, Querceto, Martella, Mazzantini, Scellini, Giammarino, Lupi, Mazzarotto, Gowran, Rovina, Santoro, Pompilio, Tesi, Parmeggiani, Regnier, Cerbai, Mack, Poggesi, Ferrantini and Coppini.

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Phosphorylation of RyR2 and CaMKIIδ in hiPSC-CMs. Western blot (WBs) analysis of Ryanodine receptors (RyR2) phosphorylation status and verification of CaMKII activity in day 90 DMD-vs Control-hiPSC monolayers. (A) Representative WB of CaMKIIδ (B) and analysis normalized for total CaMKII. (C) Representative WB analysis of (D) RyR2 sites of phosphorylation (phospho-specific anti Ser 2814 target of CaMKII and Ser 2808 target of PKA) normalized for GAPDH, *p < 0.05 in ANOVA DMD vs Controls N = 3 (differentiation runs) and n = 2–5 (gel repetitions).