Apoptosis of NK cells results in the dominance of ILC1. (A) Absolute number of liver ILC1 and NK cells in CD- or EtOH-fed mice was counted (n = 8/group). (B) Absolute number of CD4+ T, CD8+ T, and B cells in CD- or EtOH-fed mice was measured by flow cytometry (n = 4/group). (C and D) Apoptosis of liver NK cells (C) and ILC1 (D) from CD- or EtOH-fed mice was measured by using anti-annexin V antibody and 7-AAD (n = 5/group). Annexin V+ cells were identified as apoptotic cells. (E) The KEGG analysis was performed by using previously published GEO database (GSE43339). (F) Expression of Ki67 in liver ILC1 and NK cells from CD- or EtOH-fed mice was examined (n = 5/group). Two-way analysis of variance was performed in A and B. Two-tailed unpaired Student t tests were performed in C, D, and F. ns, no statistical significance. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P <.0001.