Figure 3.

Restoration of NK cells protects mice from alcohol-induced steatohepatitis. (A–F) CD- or EtOH-fed mice were injected intravenously with PBS or WT splenic CD49b⁺NK cells from Rag1^-/- mice at days 2, 6, and 10 after ethanol feeding (1 × 10⁶ cells each time). Mice were killed, and liver tissue or plasma was obtained at 6 hours after binge. The number of liver ILC1 and NK cells (A) and ratio of ILC1 to NK cells (B) were shown (n = 5–6/group). (C) Hepatic triglyceride content was measured in whole liver homogenate (n = 4–6/group). (D) Plasma concentrations of ALT and AST were measured (n = 4–7/group). (E) The mRNA level of IL6 and IL1β in liver tissue was detected (n = 4–6/group). (F) H&E or Oil Red O staining of liver section were performed (scale bars, 100 μm). Representative images are shown (n = 4/group). Two-way analysis of variance was performed in A. One-way analysis of variance was performed in B–E. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P <.0001.