Figure 5.

Liver ILC1 play an important role in alcohol-induced steatohepatitis. EtOH-fed mice were injected intraperitoneally with anti-AsGM1 antibody (50 μg per mouse) or same amount of IgG as control once at 48 hours before binge. (A) The numbers of liver ILC1 and NK cells were measured at 6 hours after binge (n = 4/group). (B) Hepatic triglyceride content was measured in whole liver homogenate (n = 18–20/group). (C) Plasma concentrations of ALT and AST were measured (n = 8–12/group). (D) H&E and Oil Red O staining of liver section were performed at 6 hours after binge (scale bars, 100 μm). Representative images are shown (n = 8/group). (E) The mRNA level of IL6 (n = 6–7/group), IL1β (n = 6–8/group), and TNF-α (n = 3/group) in liver tissue was detected. (F) Female C57BL/6 mice were exposed to CD or ethanol containing diet. Some EtOH-fed mice were injected intraperitoneally with IgG or anti-AsGM1 antibody. All mice were killed at 6 hours after binge, and the percentage and absolute number of hepatic neutrophils were measured by flow cytometry (n = 6/group). Two-way analysis of variance was performed in A. Two-tailed unpaired Student t tests were performed in B, C, and E. One-way analysis of variance was performed in F. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P <.0001.