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CAV infection blocks ISD-mediated IFN-β expression. Following incubation with 1 × 104 TCID50 CAV for 3 h, cells were transfected with or without ISD before RT-qPCR. At 6 h post ISD transfection, cells were harvested at 6 h and evaluated for the level of IFN-β mRNA using RT-qPCR. Data are presented as mean ± standard deviations from at least three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001.
