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. 2022 Oct 25;102(1):102291. doi: 10.1016/j.psj.2022.102291

Table 1.

Oligonucleotide sequences for PCR and luciferase reporter assays.

Gene Name Oligonucleotide sequences (5′-3′)
cGAS Myc-cGAS-F GGAATTCGGATGGAGGAGACCGCGGCGGGCA
Myc-cGAS-R GGGGTACCCTACACCTGGTGAAATACTGGGAATCC
CAV-VP1 mCherry-VP1-F CCGCTCGAGAGATGGCAAGACGAGCTCGCAGAC
mCherry-VP1-R GGAATTCAATCAGGGCTGCGTCCCCCAG
EGFP-VP1-F GGAATTCTATGGCAAGACGAGCTCGCAGAC
EGFP-VP1-R GGGGTACCTCAGGGCTGCGTCCCCCAG
STING Myc-STING-F GGAATTCGGATGCCCCAGGACCCGTCAACC
Myc-STING-R GGGGTACCTCAGGGGCAGTCACTGCGCAG
IRF7 Myc-IRF7-F GGAATTCGGATGGCAGCACTGGACAGCG
Myc-IRF7-R GGGGTACCTCAGTCTGTCTGCATGTGGTATTG
EGEP-IRF7-F GGAATTCTATGGCAGCACTGGACAGCG
EGEP-IRF7-R GGGGTACCTCAGTCTGTCTGCATGTGGTATTG
cGAS cGAS-luc-F CCGCTCGAGGAAAAGAGTCACCACAAAAATCTGGC
cGAS-luc-R CCCAAGCTTCGCCTCTCGGGCGGCACA
STING STING-luc-F GGGGTACCTGAACAGGGTGGGGGTACTGCTGGA
STING-luc-R CCGCTCGAGTGCCCTGTGCCTGCCTCCTGTCTG
TBK1 TBK1-luc-F GGGGTACCCTTGGAGTCCTGCTGGTCCTCTTC
TBK1-luc-R CCCAAGCTTGAGCAGCGCGCGGCGCG
IRF7 IRF3-luc-F GGGGTACCCCTTGTATTTGGGAAGGAATCTGTGC
IRF3-luc-R CCGCTCGAGGGACGGGACCACCCCGA
IFN-β IFN-β-luc-F GGGGTACCGCATCCTCCAACACCTCTTCAACAT
IFN-β-luc-R CCGCTCGAGTGGTTTACGAAGCATTGCTCAAGGT