Figure 1.

TFR reduce CXCR4-tropic HIV replication in TFH. (A) A representative gating strategy for sorting TFH (CD3+CD8-CXCR5+CD25^lo) and TFR (CD3+CD8-CXCR5+CD25^hi) from CD4-enriched disaggregated tonsil cells. (B) FoxP3 expression in TFH and TFR populations (n=4). (C) Representative gating strategy to determine CXCR5⁺FoxP3⁺ expression in TFH and TFR populations. (D) Percentages of CXCR5⁺FoxP3⁺ cells gated as TFH or TFR (n=4). (E) Representative gating strategy for detection of VPD⁺ TFH and GFP expression in TFH cocultures. TFH were spinoculated with CXCR4-tropic HIV GFP reporter virus, labeled with VPD, and cultured for five days at a ratio of 1:1 with uninfected, unlabeled TFH (control) or TFR in media supplemented with 10 IU/ml IL-2 and 5 μM saquinavir (n=11). After five days, percentages of GFP⁺VPD⁺ TFH (F) and GFP-MFI (G) of GFP⁺VPD⁺ TFH were determined. Statistical analyses were performed using Wilcoxon matched-pairs tests using Graphpad Prism v8 and significance indicated: **p<0.01; ***p<0.001.