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PCR amplification products analysis. (A) One-kilobase DNA ladder (Life Technologies). Amplication products were generated by using primers for tia-specific sequence of ETEC strain H10407 (B), eae-specific sequence of EPEC (C), and ipaC-specific sequence of S. flexneri SC301 (D). DNA to be amplified was released by boiling from ETEC strain H10407 (B, lane 1), EPEC strain E2348/69 (C, lane 1), EIEC strain E12860/0 (D, lane 1), strain LF82 (lane 2), and S. flexneri SC301 (D, lane 3).
