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. 1999 Sep;67(9):4499–4509. doi: 10.1128/iai.67.9.4499-4509.1999

FIG. 5.

FIG. 5

Effects of eukaryotic cell inhibitors on association with (A and B) and invasion of (C and D) HEp-2 cells. HEp-2 cell monolayers were pretreated with cytochalasin D or colchicine for 30 min before the assay. Infections were performed for 3 h in the presence of the inhibitors. Cell-associated bacteria were quantified after the 3-h infection period. Invasion was determined after gentamicin treatment for an additional hour. Results are expressed as cell-associated or intracellular bacteria relative to those obtained without inhibitor, taken as 100%. ■, strain LF82; Created by potrace 1.16, written by Peter Selinger 2001-2019 , EIEC strain E12860/0; ░⃞, EPEC strain E2348/69. Each value is the mean of at least three separate experiments.