TABLE 4.
Survival of strain LF82 within HEp-2 and Intestine-407 cells
| Gentamicin treatment period (h) | Intracellular
survival (mean ± SD [n ≥ 3])
|
|||||
|---|---|---|---|---|---|---|
| HEp-2
|
Intestine-407
|
|||||
| CFU × 105/wella | % Relative CFUb | % Cellsc | CFU × 105/well | % Relative CFU | % Cells | |
| 1 | 690.0 ± 42.4 | 100 | 100 | 203.5 ± 60.1 | 100 | 100 |
| 2 | 1,240.0 ± 56.6 | 179.8 ± 5.0 | NDd | 283.5 ± 65.8 | 140.7 ± 9.2 | ND |
| 4 | 1,530.0 ± 183.9 | 223.0 ± 40.4 | ND | 198.0 ± 45.3 | 98.3 ± 6.8 | ND |
| 6 | 1,720.0 ± 537.4 | 247.4 ± 62.7 | 99.8 | 218.5 ± 34.6 | 109.6 ± 15.4 | 157.6 |
| 24 | 1,685.0 ± 459.6 | 242.6 ± 51.7 | 114.5 | 163.3 ± 34.9 | 81.3 ± 6.9 | 159.3 |
a
Number of CFU recovered from lysed monolayers.
b
Percent intracellular bacteria relative to the number after 1 h of gentamicin treatment, defined as 100%.
c
Percent epithelial cells remaining attached to the plates relative to the number after 1 h of gentamicin treatment, defined as 100%.
d
ND, not determined.