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Immunoblot of the total protein extracts from S. mutans GS5(gtfB)::pSIV2 (lane 1), S. mutans GS5(gtfB)::pSIV2-SGPAN (lane 2), and S. mutans BCH 150 (lane 3) grown in SMM–1% sucrose at 37°C to the early stationary growth phase. The bands corresponding to glucan-binding protein A (GbpA) and pyruvate kinase (PYK) are indicated with arrows.
