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Immunoblot of total protein extracts from S. mutans GS5(gtfB)::pSIV2-SGPAN grown in 5 ml of SMM with 1% sucrose (lane 1), 1% sucrose plus 0.1% glucose (lane 2), 1% sucrose plus 0.4% glucose (lane 3), and 1% glucose (lane 4) at 37°C to the early stationary growth phase. The bands corresponding to glucan-binding protein A (GbpA) and pyruvate kinase (PYK) are indicated with arrows.
