TABLE 1.
Effects of the environment on the cellular distribution of SGP
| Growth conditionsa | SGP, DUb (%)
|
||
|---|---|---|---|
| Total | Membrane associated | Cytoplasmic | |
| 43°C, mid-log phase | 368 ± 16 | 239 ± 9 (65) | 128 ± 24 (35) |
| 43°C, stationary phase | 408 ± 36 | 244 ± 10 (60) | 164 ± 26 (40) |
| 37°C, mid-log phase | 150 ± 15 | 33 ± 20 (28) | 117 ± 26 (72) |
| 37°C, pH 5.5, mid-log phase | 390 ± 23 | 244 ± 25 (63) | 146 ± 29 (37) |
| 37°C, mid-log phase, 50 μM mycophenolic acid | 263 ± 19 | 128 ± 5 (49) | 135 ± 23 (51) |
| 37°C, mid-log phase, 250 μM psicofurarine | 252 ± 15 | 127 ± 3 (51) | 125 ± 14 (49) |
a
Growth was in SMM–1% glucose at pH 7.0 unless indicated otherwise.
b
Means and standard deviations from three independent experiments are shown. The values (arbitrary DU) were obtained by scanning the immunoblots as shown in Fig. 2.