Fig. 4.

TATE induces HNSCC metastasis. A-B, Culture medium from M0 macrophage (a baseline control), M2 macrophage (a positive control), and activated eosinophil were added to HUVEC assay. Scale bar: 100um. Number of junction and enclosed space were quantified at low power field (40X). Arterisk: p<0.05. C-E, Representative images for TATE-poor and TATE-enriched ROI. H&E staining (left), CD31 IHC staining (middle), and automated identification and quantification of CD31-positive area (Right). Scale bar: 200um. Arrowhead: eosinophil. Red area: automatically identified CD31-positive area. Green: automatically identified CD31-negative area. Arterisk: p<0.05. F, GSEA analysis of RNA sequencing database from TVGH HNSCC cohort (65 samples from 23 patients) for angiogenesis pathway. NES: normalized enrichment score. FDR: false discovery rate.