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A, Targeting strategy for creating Gcg-Cre rats. B, Southern blot restriction enzyme strategy and confirmation (C) in nine F1 Gcg-Cre+/− rats. Genomic DNA digestion via BgIII, 5′ probe yields two potential DNA fragments (6.3 kb targeted allele, 15.7 kb WT allele). DNA digestion via EcoNI, iCre probe (3′) yields one DNA fragment (4.3 kb targeted allele, no band for WT allele).
