Skip to main content
. 2022 Nov 8;66:101631. doi: 10.1016/j.molmet.2022.101631

Figure 4.

Figure 4

Gcg and iCre mRNA expression (RNAscope) in the olfactory bulb in adult Gcg-Cre/tdTom reporter rats. A, sagittal section through the olfactory bulb. The boxed inset (b) is shown at higher magnification in panel B. Within the inset, a white arrow points out a representative cell immunolabeled for red fluorescent protein (RFP = tdTom-positive). The 4 panels in the lower left of panel A show Gcg mRNA expression (green) in the RFP-positive cell. DAPI nuclear labeling is shown in blue. B, Gcg mRNA is colocalized to one RFP-positive cell (arrow), but not to two other RFP-positive cells in the same region. C, iCre mRNA (green) is colocalized in several tdTom-positive cells within the periglomular olfactory bulb (yellow arrows), but is not detected in other tdTom-positive cells (open arrows). D, lower-magnification view of tdTom labeling in the olfactory bulb. The boxed region (c) is shown at higher magnification in panel C. Scale bars are in microns.