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. 2022 Nov 8;66:101631. doi: 10.1016/j.molmet.2022.101631

Figure 6.

Figure 6

Central patterns of tdTom immunoperoxidase labeling in adult Gcg-Cre/tdTom reporter rats. A, B, coronal sections through two rostrocaudal levels of the cNTS and IRt. cc, central canal. C, parasaggital section through the caudal medulla, depicting tdTom-positive neurons and extensive dendritic branching in the cNTS and IRt. Inset shows the boxed region that is enlarged in panel C. 4V, fourth ventricle; 10, dorsal motor nucleus of the vagus; 12, hypoglossal nucleus; AP, area postrema; py, pyramidal tract; pyx, pyramidal decussation.D, paraflocculus (pf) of cerebellum (shown at lower magnification in the inset). E, inferior colliculus (IC, shown at lower magnification in the inset). An example of a tdTom-positive neuron within the inferior colliculus is shown at higher magnification in the inset in lower center of panel E. CA, cerebral aqueduct.F, labeled pyramidal neurons within the piriform cortex (pir). The inset in panel F shows a lower-magnification view of the piriform cortex and nearby basolateral amygdala (BLA). See Figure 7 for additional images of BLA labeling. dHC, dorsal hippocampus; st, stria terminalis.G, ventral orbital (orb) and piriform cortex (shown at lower magnification in the inset). fr, frontal cortex; rf, rhinal fissure. Scale bars are in microns, except in the panel C inset.