Table 1.

Primer sequences and predicted PCR product sizes.

Primer	Sequence (5′-3′)	Tm (°C)	PCR product size (bp)
Gcg-WT-F	GTCTCTGACCCAGTCTCCAGCACTA	62	WT:454
Gcg-WT-R	ATGTATTCGTCCGCATGCAAAGCAG	63
Gcg-Mut-F	CGATGGTGGGAGAATGCTGATCCAC	63	Mut:581
Gcg-WT-R	ATGTATTCGTCCGCATGCAAAGCAG	63

Primer sequences and predicted PCR product sizes for detection of knockin sequence integrated into the rat genome. The use of two primer sets permits identification of three genotypes (i.e., +/+, +/−, and −/− for the Mut Gcg allele encoding iCre) via gel electrophoresis. WT, wild type allele; Mut, mutant targeted allele.