FIG. 3.

Effect of Ca²⁺ on the interaction between GST-Fbe and Fg. The binding of GST-Fbe and of Fbe to Fg was measured at different concentrations (conc.) of Ca²⁺ essentially as described in the legend to Fig. 2A. Likewise, the binding of Fg to GST-Fbe and to Fbe was measured at different concentrations of Ca²⁺. All proteins were dialyzed against EDTA to remove endogenous Ca²⁺. Symbols: ■, binding of Fg to GST-Fbe; □, binding of Fg to Fbe; ●, binding of GST-Fbe to Fg; ○, binding of Fbe to Fg. The experiment shown is representative of at least three experiments.