FIG. 5.

Competition in a capture ELISA with ClfA or GST-Fbe. Microtiter plate wells were coated with GST-Fbe or ClfA and then with BSA. The indicated concentrations (conc.) of GST-Fbe or ClfA as an inhibitor were added immediately before the addition of Fg. The relative amount of bound Fg was determined with HRP-conjugated antibodies against Fg. Symbols: □, GST-Fbe inhibition of Fg binding to GST-Fbe; ○, ClfA inhibition of Fg binding to ClfA; ▵, inability of GST-Fbe to inhibit Fg binding to ClfA. The experiment shown is representative of at least three experiments.