FIG. 6.

(a) Western blot of separated Fg chains. Fg from SDS-PAGE was transferred to nitrocellulose filters. The filters were probed with either GST-Fbe followed by rat anti–GST-Fbe antibodies or ClfA followed by rat anti-ClfA antibodies and, in both cases, by anti-rat IgG antibodies conjugated with HRP. Lanes (Coomassie blue-stained gel in lanes 1 and 2 and Western blot strips in lanes A to D): 1, molecular size markers (94, 67, and 43 kDa, from the top); 2, α, β, and γ chains of Fg; A, membrane probed with GST-Fbe and anti–GST-Fbe serum; B, as in A, but without GST-Fbe; C, membrane probed with ClfA and anti-ClfA serum; D, as in C, but without ClfA. (b) Western blot of purified Fg chains. Purified α, β, and γ chains (lanes 2, 3, and 4, respectively) from SDS-PAGE were transferred to nitrocellulose filters. (B) The filters were probed with GST-Fbe followed by rat anti–GST-Fbe antibodies and anti-rat IgG antibodies conjugated with HRP. (C) As in B, but without GST-Fbe. (A) Coomassie blue-stained gel. Lanes 1, molecular size markers.