TABLE 1.
Primers of virulence and antibiotic resistance genes, annealing temperatures and size of amplicons
| Gene | Primer sequences (5′–3′) | Annealing temperatures (°C) | Product size (bp) |
|---|---|---|---|
| 23S rRNA |
TTAGCTAATGTTGCCCGTACCG TAGTAAAGGTCCACGGGGTCGC |
46 | 485 |
| recR |
GATGATCCTGACTTTG TCTCCTATTTTTACCC |
45 | 584 |
| dnaJ |
AAGGCTTTGGCTCATC CTTTTTGTTCATCGTT |
46 | 720 |
| wlaN |
TTAAGAGCAAGATATGAAGGTG CCATTTGAATTGATATTTTTG |
46 | 672 |
| Virbll |
TCTTGTGAGTTGCCTTACCCCTTTT CCTGCGTGTCCTGTGTTATTTACCC |
53 | 494 |
| cdtC |
CGATGAGTTAAAACAAAAAGATA TTGGCATTATAGAAAATACAGTT |
47 | 182 |
| cdtB |
CAGAAAGCAAATGGAGTGTT AGCTAAAAGCGGTGGAGTAT |
51 | 620 |
| cdtA |
CCTTGTGATGCAAGCAATC ACACTCCATTTGCTTTCTG |
49 | 370 |
| flaA |
AATAAAAATGCTGATAAAACAGGTG TACCGAACCAATGTCTGCTCTGATT |
53 | 585 |
| cadF |
TTGAAGGTAATTTAGATATG CTAATACCTAAAGTTGAAAC |
45 | 400 |
| pldA |
AAGCTTATGCGTTTTT TATAAGGCTTTCTCCA |
45 | 913 |
| ciaB |
TTTTTATCAGTCCTTA TTTCGGTATCATTAGC |
42 | 986 |
| ceuE |
CCTGCTACGGTGAAAGTTTTGC GATCTTTTTGTTTTGTGCTGC |
48.9 | 793 |
| cgtB |
TAAGAGCAAGATATGAAGGTG GCACATAGAGAACGCTACAA |
49.9 | 561 |
| tet(O) |
GCGTTTTGTTTATGTGCG ATGGACAACCCGACAGAAG |
54 | 559 |
| cmeB |
TCCTAGCAGCACAATATG AGCTTCGATAGCTGCATC |
54 | 241 |
| bla OXA‐61 |
AGAGTATAATACAAGCG TAGTGAGTTGTCAAGCC |
54 | 372 |
| aphA‐3‐1 |
TGCGTAAAAGATACGGAAG CAATCAGGCTTGATCCCC |
54 | 701 |