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. Author manuscript; available in PMC: 2022 Nov 21.
Published in final edited form as: Biochemistry. 2017 Jul 7;56(28):3619–3631. doi: 10.1021/acs.biochem.7b00114

Figure 2.

Figure 2.

tRNAHis and total tRNA binding are unaffected by Y454S. A) Y454S and HARS tRNAHis binding forward curve. HARS (circles) and Y454S (triangles) varied from 0.1 to 30 μM. 32P-tRNAHis concentration was 30 nM. B) Competition Plot where transcribed tRNAHis bound to either HARS or Y454S was competed by increasing concentrations of total tRNA purified from human placenta. 32P-tRNAHis concentration was 30 nM, enzyme (HARS (circles) and Y454S (triangles)) was 5 μM and placental tRNA varied from 0 to 100 μM. Values reported are the mean ± standard error of two independent experiments.