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. Author manuscript; available in PMC: 2022 Nov 21.
Published in final edited form as: Biochemistry. 2017 Jul 7;56(28):3619–3631. doi: 10.1021/acs.biochem.7b00114

Figure 4.

Figure 4.

A. Molar mass distributions determined by sedimentation velocity, showing the PCSA-Monte Carlo distributions for HARS WT (black), HARS Y454S (red), and HARS R362H (blue). Molar masses are in excellent agreement with a dimer of each species. B. Frictional ratio anisotropies for HARS WT (black), HARS Y454S (red), and HARS R362H (blue) as a function of the sedimentation coefficient. Pseudo-3D plots derived from PCSA-Monte Carlo analyses of sedimentation velocity experiments. The right vertical axis indicates the partial concentration in terms of color density. All three samples show remarkable similarity.