Figure 6.

Human fibroblasts from patients with the Y454S mutation demonstrate less de novo protein synthesis at elevated temperatures than wild type cells. Scatter plot showing ³H-histidine incorporation into total cellular protein (values normalized to cellular DNA), Y454S cells (circles) and wild type cells (squares). Values shown include three replicates each for three Y454S cell lines and three replicates each for six wild type cell lines (culture temperature 37 °C solid symbols, 43 °C open symbols). Bars indicate the mean +/− SEM. Statistical significances were determined by Welch’s t-test.