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Pre-steady state rate for amino acid activation.
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| Rate of amino acid activation | ||
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| Reactants | Rate of exponential phase (s−1) | Rate of linear phase k1 (s−1) |
| WT HARS (n=3) | 19 ± 15 | 3 ± 1a |
| WT HARS + tRNAHis (n=2) | 47 ± 21 | 9.1 ± 0.7a |
| Y454S (n=2) | 18 ± 6 | 2.8 ± 0.7b |
| Y454S HARS + tRNAHis (n=2) | 21 ± 5 | 6.4 ± 0.6b |
Values reported are the mean ± standard error of n independent experiments.
Significantly different at p<0.05.
Significantly different at p<0.01 (extra sum-of-squares F test).
