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. Author manuscript; available in PMC: 2022 Nov 21.
Published in final edited form as: Biochemistry. 2017 Jul 7;56(28):3619–3631. doi: 10.1021/acs.biochem.7b00114

Table 4.

PCSA – Monte Carlo results (straight-line parameterization) from the SV experiment.

Species Molar mass (kDa) D (× 10−7 cm/sec2) s (× 10−13 sec) f/f0 RMSD
WT 118.5 (111.6, 125.5) 4.54 (4.43, 4.66) 5.67 (5.48, 5.85) 1.44 (1.43, 1.45) 0.002484
Y454S 116.5 (108.6, 124.3) 4.62 (4.46, 4.77) 5.66 (5.47, 5.84) 1.43 (1.41, 1.44) 0.002468
R362H 117.7 (109.8, 125.5) 4.55 (4.40, 4.71) 5.63 (5.45, 5.81) 1.44 (1.43, 1.46) 0.002825

Values in parenthesis are 95% confidence intervals from the Monte Carlo analysis. The theoretical molar mass of HARS WT dimer is 114.9 kDa, indicating the observed species is a dimer. The residual mean square deviation (RMSD) of the fit is shown in units of absorbance at 230 nm.