|
Antibodies
|
|
| BRAF (H-145) rabbit polyclonal |
Santa Cruz Biotechnology |
cat# sc-9002; RRID:AB_2067494 |
| HaloTag mouse monoclonal |
Promega |
cat# G9211; RRID:AB_2688011 |
| NanoLuc (Nluc) luciferase antibody |
R&D Systems |
cat# MAB100261 |
| FLAG M2 antibody |
Millipore/Sigma |
cat# F3165; RRID:AB_439685 |
| FLAG (L5) rat monoclonal antibody |
BioLegend |
cat# 637304; RRID:AB_1134267 |
| MEK1 mouse monoclonal |
BD Biosciences |
cat# 61022; RRID:AB_397528 |
| pS217/221-MEK rabbit polyclonal |
Cell Signaling Technology |
cat# 9121; RRID:AB_331648 |
| β-Tubulin mouse monoclonal |
Santa Cruz Biotechnology |
cat# sc-5274; RRID:AB_2288090 |
| α-Tubulin antibody, mouse monoclonal |
Millipore Sigma |
cat# T6199; RRID:AB_477583 |
| Pan-RAS rabbit polyclonal antibody |
Cell Signaling Technology |
cat# 3965; RRID:AB_2180216 |
| Alexa Fluor 568 goat anti-rat polyclonal antibody |
Thermo Fisher |
cat# A-11077; RRID:AB_2534121 |
| E-Cadherin, rat monoclonal antibody |
Invitrogen |
cat# 14-3249-82; RRID:AB_1210458 |
|
|
Chemicals
|
|
| Platelet Derived Growth Factor (PDGF) |
Millipore/Sigma |
cat# SRP3229 |
| X-tremeGENE 9 |
Roche/Sigma |
cat# 06365809001 |
| Collagen, Human Placenta Type IV |
Millipore/Sigma |
cat# C7521 |
| Doxycycline ((Z)-4-Hydroxytamoxifen) |
Millipore Sigma |
cat# H7904 |
| Paraformaldehyde |
EM Sciences |
cat# 15710 |
| Coelenterazine-h |
Promega |
cat# S2011 |
|
|
Critical Commercial Assays
|
|
| CellTiter-Glo 2.0 |
Promega |
cat# G9241 |
| QuickChange II Kit Agilent |
Agilent |
cat# 200523 |
| NanoBRET Kit |
Promega |
cat# N1661 |
|
|
Deposited Data
|
|
| Raw data files |
This paper |
Mendeley Data: https://doi.org/10.17632/d89bbp4k2k.1
|
|
|
Experimental Models: Cell Lines
|
|
| 293FT (human) |
ATCC |
cat# PTA-5077, RRID:CVCL_6911 |
| Phoenix-Eco (human) |
ATCC |
cat# CRL-3214; RRID:CVCL_H717 |
| NIH 3T3 (mouse) |
ATCC |
cat# CRL-1658; RRID:CVCL_0594 |
| COS7 |
ATCC |
cat# CRL-1651; RRID:CVCL_0224 |
| DU1473 |
NCI-RAS Initiative |
N/A |
|
|
Recombinant DNA
|
|
| pEGFP-C1-BRAF-CRD mutants |
This paper |
N/A |
| pCMV5-BRAFREG-mutants-Halo |
This paper |
N/A |
| pCMV5-NanoLuc-BRAFCAT mutants |
This paper |
N/A |
| pCMV5-NanoLuc-CRAFCAT
|
This paper |
N/A |
| pCMV5-CRAFREG-Halo |
This paper |
N/A |
| pLenti-KRASG12V
|
NCI-RAS Initiative |
N/A |
| pCMV5-Venus-KRASG12V
|
NCI-RAS Initiative |
N/A |
| pLHCX-BRAFFL-RLuc8 |
Terrell et al., 2019
|
N/A |
| pLHCX-BRAFFL CRD mutants-RLuc8 |
This paper |
N/A |
| pLHCX-BRAFREG CRD-RLuc8 |
Terrell et al., 2019
|
N/A |
| pLHCX-BRAFREGCRD mutants-RLuc8 |
This paper |
N/A |
| pCS2+-BRAFFL CRD mutants |
This paper |
N/A |
| pBabe-Puro-FLAG-BRAF |
Freeman et al., 2012 |
N/A |
| pBabe-Puro-FLAG-BRAF mutants |
This paper |
N/A |
| pBabe-Puro-FLAG-CRAF |
Freeman et al., 2012 |
N/A |
| pBabe-Puro-FLAG-CRAF mutants |
This paper |
N/A |
| pEGFP-C1-CRAF-CRD mutants |
This paper |
N/A |
|
|
Software and Algorisms
|
|
| GraphPad Prism |
GraphPad |
https://www.graphpad.com
|
| ImageJ |
ImageJ |
https://imagej.nih.gov/ij/
|
