KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit 5-HT (Serotonin) Antibody | ImmunoStar | Cat#20080, RRID: AB_572263 |
| Mouse Monoclonal Anti-Chondroitin Sulfate antibody | Sigma-Aldrich | Cat#C8035, RRID: AB_476879 |
| Rabbit Doublecortin (DCX) Antibody | Cell Signaling Technology | Cat#4604, RRID:AB_561007 |
| Mouse Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody | Sigma-Aldrich | Cat# G3893, RRID:AB_477010 |
| Rat Ki-67 monoclonal antibody (SolA15) | Invitrogen | Cat#14-5698-82, RRID:AB_10854564 |
| Goat Nurr1 Polyclonal antibody | R & D Systems | Car#AF2156, RRID:AB_2153894 |
| Guinea pig VGluT2 polyclonal antibody | Sigma-Aldrich | Cat#AB2251-I, RRID:AB_2665454 |
| Rabbit Homer1 polyclonal antibody | Sigma-Aldrich | Cat#ABN37, RRID:AB_11214387 |
| Mouse Bcl-2 (C-2) antibody | Santa Cruz | Cat#sc-7382, RRID:AB_626736 |
| Rabbit Iba1 antibody | FUJIFILM Wako | Cat#019-19741, RRID:AB_839504 |
| Mouse AKT antibody | Proteintech | Cat#60203-2-lg, RRID:AB_10912803 |
| Rabbit ERK1/2 antibody | Proteintech | Cat#16443-1-AP, RRID:AB_10603369 |
| Rabbit p-Akt (Ser473) antibody | Cell Signaling Technology | Cat#4060S, RRID:AB_2315049 |
| Mouse GAPDH antibody | Proteintech | Cat# 60004-1-Ig, RRID:AB_2107436 |
| Rabbit p-ERK1/2 (Thr202/Tyr204) antibody | Cell Signaling Technology | Cat# 4370, RRID:AB_2315112 |
| VECTASTAIN ABC-Peroxidase Kit | Vector Laboratories | Cat# PK-4001, RRID:AB_2336810 |
| Chemicals, peptides, and recombinant proteins | ||
| ISP | CS-Bio | N/A |
| Scrambled ISP (SISP) | CS-Bio | N/A |
| LY294002 | Sigma-Aldrich | Cat# 19-142 |
| PD98059 | Sigma-Aldrich | Cat# P215 |
| OA-Hy | Sigma-Aldrich | Cat# 444244 |
| Chondroitinase ABC | Sigma-Aldrich | Cat# C2905 |
| Aggrecan | Sigma-Aldrich | Cat# A1960 |
| Biotin Dextran Amine | Invitrogen | Cat# D1956 |
| Deposited data | ||
| RNA-seq data | This paper | GEO:GES168934 |
| Experimental models: Organisms/strains | ||
| Mouse: Ptprstm1b(KOMP)Mbp/Ptprs+ | KOMP | MGI Cat# 5797751, RRID:MGI:5797751 |
| Mouse: C57BL/6-Tg(Nes-cre/ERT2)KEisc/J | The Jackson Laboratory | IMSR Cat# JAX:016261, RRID:IMSR_JAX:016261 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J | The Jackson Laboratory | IMSR Cat# JAX:007909, RRID:IMSR_JAX:007909 |
| Mouse: B6N(B6J)-Tg(CAG-Flpo) 1Afst/Mmucd | MMRRC | MMRRC Cat# 036512-UCD, RRID:MMRRC_036512-UCD |
| Oligonucleotides | ||
| Primers: Hprt1 | IDT | F: TGA TAG ATC CAT TCC TAT GAC TGT AGA R: AAG ACA TTC TTT CCA GTT AAA GTT GAG |
| Primers: Igfn1 | Applied Biosystems | Assay ID: Mm00617360_m1 |
| Primers: Penk | IDT | F: AAC ACC GGC AAT GGA CTG R: AAA CTC GCC TGG ATT TTG G |
| Primers: Rasgef1c | Applied Biosystems | Assay ID: Mm01288803_m1 |
| Primers: Dact2 | Applied Biosystems | Assay ID: Mm00555888_m1 |
| Primers: Grm2 | Applied Biosystems | Assay ID: Mm01235831_m1 |
| Primers: Cited4 | IDT | F: CCG AGA ACA CCT GCC TTG R: AGC GAG ACC CAA CTG TCA TC |
| Primers: Nurr1 | IDT | F: TCA GAG CCC ACG TCG ATT R: TAG TCA GGG TTT GCC TGG AA |
| Primers: Sema4d | IDT | F: AAG TGG GTG CGC TAC AAT G R: GGG CCT CAC TGT CGA TAC AC |
| Primers: Bcl2 | IDT | F: GTA CCT GAA CCG GCA TCT G R: GGG GCC ATA TAG TTC CAC AA |
| Primers:MMP2 | IDT | F: CAG GGA ATG AGT ACT GGG TCT ATT R: ACT CCA GTT AAA GGC AGC ATC TAC |
| Primers:GAPDH | IDT | F: TGC CAA ATA TGA TGA CAT CAA GAA R: GGA GTG GGT GTC GCT GTT G |
| Software and algorithms | ||
| Stereo Investigator | MBF Bioscience | RRID:SCR_017667 |
| ImageJ | NIH | RRID:SCR_003070 |
| MATLAB | Mathworks | RRID:SCR_001622 |
| SigmaPlot | SigmaPlot | RRID:SCR_003210 |